This document describes an improved method for the simultaneous measurement of sirolimus and tacrolimus concentrations in blood, in which the drugs are first isolated from whole-blood specimens by solid phase extraction and then analyzed by liquid chromatography and electrospray-mass spectrometry.
This document details an improved analytical method for measuring the immunosuppressants sirolimus and tacrolimus in whole-blood specimens. The method utilizes solid phase extraction followed by analysis via liquid chromatography and electrospray ionization mass spectrometry (LC/ESI-MS). The procedure is designed to be semi-automated to increase sample throughput and productivity in clinical laboratories.
| Limit of Detection (Sirolimus) | 0.38 ug/L |
|---|---|
| Analytical column | Restek Allure C18, 30 mm long x 3.2 mm id |
| Flow rate | 0.5 mL/min |
| Temperature | 75 °C |
Yes, both drugs are measured using the same extraction and LC/MS conditions.
The flow rate is set to 0.5 mL/min.