Agilent Technologies 2100 Bioanalyzer Apoptosis Analysis Application Note

About this manual

The document presents an application note detailing a combined measurement of Annexin V, active-caspase-3, and DNA laddering analysis on the Agilent 2100 bioanalyzer platform. This approach allows for quick confirmation and quantitation of apoptosis in cell populations using the Cell Fluorescence LabChip kit and the DNA LabChip kit.

What's inside

• Introduction
• Apoptosis Overview
• Experimental Protocol
• Fast protocol for DNA fragment purification
• UV measurements
• Annexin V apoptosis measurements
• Results and Discussion
• DNA laddering analysis

Frequently asked questions

What kits are used for the combined apoptosis measurement?

The protocol uses the Cell Fluorescence LabChip kit for Annexin V and active-caspase-3, and the DNA LabChip kit for laddering analysis.

What is the recommended centrifugation speed for the fast DNA purification protocol?

The protocol requires centrifuging the supernatant at 13000 g for 5 minutes.

What is the concentration range specification for the DNA 12000 LabChip kit?

The kit is specified for a concentration range of 0.5-50 ng/pL.

What size intervals confirm apoptosis in the DNA laddering assay?

Apoptosis is confirmed by fragmentation of chromosomal DNA in 180-200bp intervals.