This document introduces the optimization of Agilent ZORBAX Eclipse XDB-C18 column for ultra-rapid HPLC separations. The parameters optimized include column, separation time, mobile-phase re-equilibration, and baseline drift.
This document describes the optimization of a liquid chromatography method using Agilent ZORBAX Eclipse XDB-C18 columns. It demonstrates how switching to this specific column and adjusting gradient times can significantly reduce run time from 3 minutes to under 1 minute while improving baseline stability.
| Column | ZORBAX Eclipse XDB-C18, 2.5 µm |
|---|---|
| Flow Rate | 4 mL / min |
| Detection Wavelength | 220 nm or 268 nm |
The run time is reduced from 3 minutes to just over 1 minute.
Reducing the gradient time from 2 minutes to 1 minute reduces the run time to less than 1 minute.