Agilent 2100 Bioanalyzer Application Note: Analysis of His-Tag Removal from Recombinant Proteins

This paper describes the use of the Agilent 2100 bioanalyzer to analyze the efficiency and kinetics of the cleavage process for the removal of His-tags from recombinant proteins. Data on the processing of 6xHis-tagged GFP (Green Fluorescent Protein) and IL-1β (Interleukin-1 beta) proteins is presented here as part of a comprehensive analysis of the TAGZyme system1.

Brand: Agilent
Document type: Application Note
Language: English
Pages: 4
File format: PDF
File size: 256 KB
Published: 03 July, 2012
Updated: 30 September, 2023
Model: 2100 Bioanalyzer
MD5 checksum: 4C068DB11F9E0AD5000263AAD246FA48
PDF: Agilent 2100 Bioanalyzer Application Note: Analysis of His-Tag Removal from Recombinant Proteins PDF

About this manual

The document details an analysis of the TAGZyme system, which uses dipeptidyl aminopeptidase (DAPase) for efficient exoproteolytic cleavage of N-terminal tags like the 6xHis tag. The study focuses on determining the efficiency and kinetics of this process.

What's inside

Abstract
Introduction
Results and Discussion
Kinetic data from DAPase cleavage
Conclusion

Frequently asked questions

How long does complete cleavage typically take under standard conditions?

Target protein cleavage is typically complete within 30 minutes under standard conditions.

How long does it typically take for target protein cleavage to be complete under standard conditions?