This paper presents seven examples of pharmaceutical analyses employing ZORBAX Eclipse Plus C8 columns. Eclipse Plus C8 is an excellent starting point for method development because of its excellent peak shape for bases, acids, and neutrals. Excellent peak shape contributes to better peak resolution. Eclipse Plus C8 exhibits different selec- tivity compared to Eclipse XDB C8 and Eclipse Plus C18. The shorter aliphatic C8 chain is less retentive than a C18 chain and can significantly cut analysis time compared to C18. Additionally, there is a wide variety of column con- figurations available for all analytical, rapid-resolution (RR), and high-throughput analyses. Several of these examples are scaled down from analytical methods to RR methods by substituting the longer column with a RR column. This combination of excellent peak shape and scalability to high-throughput methods make Eclipse Plus C8 a valuable tool to increase productivity wherever HPLC is used.
This document presents seven examples of pharmaceutical analyses employing ZORBAX Eclipse Plus C8 columns. It highlights that these columns offer excellent peak shape for bases, acids, and neutrals, serving as an ideal starting point for method development due to their superior resolution compared to other stationary phases like Eclipse XDB C8.
The text details the benefits of using shorter aliphatic C8 chains over C18 chains to significantly reduce analysis time while maintaining selectivity. It also explores Rapid Resolution (RR) methods, showing how substituting analytical-sized columns with shorter, smaller-particle RR columns can cut analysis time in half and improve sensitivity without increasing system pressure beyond standard operating ranges.
Yes, the shorter aliphatic chain of the Eclipse C8 has less hydrophobic interaction with analytes, allowing it to cut analysis time in half compared to Eclipse Plus C18.
Eclipse Plus C8 is available in 1.8, 3.5 and 5 um particle sizes for all analytical, high-resolution, and fast LC analyses.