This work demonstrates that Eclipse Plus C18 can be used for high-performance liquid chromatography (HPLC) separation of difficult to separate nitrogen-containing compounds without the use of triethylamine (TEA), which provides a new method for separating such compounds. In addition, the low end-group activity of Eclipse Plus C18 makes it a preferred HPLC column for analysis of pharmaceuticals and other amine-containing compounds.
This document addresses peak tailing issues in reversed-phase HPLC when analyzing basic and nitrogen-containing compounds, such as pharmaceuticals. It explains that traditional solutions like lowering pH, increasing buffer strength, or adding triethylamine (TEA) can introduce selectivity changes, resolution issues, or column contamination.
The text introduces the Agilent Eclipse Plus C18 column as a superior alternative. Due to its proprietary silica treatment and improved bonding, this column minimizes silanol activity, allowing for excellent peak shape and efficiency for amine-containing analytes without the need for mobile phase modifiers like TEA.
| Column Dimensions (Long) | 4.6 x 75 mm |
|---|---|
| Particle Size | 3.5 um |
| Part Number (Long) | 959933-902 |
| Part Number (Short) | 959963-902 |
Triethylamine acts as a competing base to reduce the availability of stationary phase silanols, which reduces peak tailing for basic compounds.
It adds an additional component to the mobile phase, alters the column in a way not easily reversed, and can cause peak tailing issues in LC-MS.
It utilizes a proprietary silica treatment and improved bonding to minimize silanol activity, providing excellent peak shape without TEA.