This document compares the performance differences in analyzing vitamins B2 and B6 using liquid chromatography and fluorescence detection. It compares the Agilent 1100 Series LC system with the Agilent 1200 Series Rapid Resolution LC system. The new column technology significantly improves performance, including faster run time, lower detection limit, and better resolution.
This document compares the performance of the Agilent 1100 Series LC system with the newer Agilent 1200 Series Rapid Resolution LC (RRLC) system. The comparison focuses on the analysis of vitamins B2 and B6 using fluorescence detection, demonstrating improvements in speed, resolution, and limit of detection (LOD) achieved with the new system and sub-2-micron particle columns.
The text details the specific equipment used, including the Agilent 1200 Series binary pump, autosampler, thermostat, and fluorescence detector. It presents chromatographic conditions and results showing that the 1200 Series system reduces run time from 10 minutes to approximately 3 minutes while significantly lowering the LOD for vitamin B2 from 20 pg to 1.1 pg.
| Column particle size | 1.8 pm |
|---|---|
| Column dimensions | 50 x 2.1 mm |
| Column temperature | 30°C |
| Flow rate | 0.7 mL/min |
| Run time | 3 min |
| LOD for Vitamin B2 | 1.1 pg |
| LOD for Vitamin B6 | < 25 pg |
| Resolution for Vitamin B6 | > 5 |
The run time is only about 3 minutes.
The run time is only about 3 minutes when using the new column technology.
The limit of detection for vitamin B2 is 1.1 pg with a signal-to-noise ratio of 2.
The resolution for vitamin B6 is greater than 5.