This application describes the use of Agilent ZORBAX Rapid Resolution (RR) and Rapid Resolution High Throughput (RRHT) technology to separate a variety of nitrogen-containing drugs used to treat heart disease. This method can quickly achieve the three main goals of speed, resolution, and sensitivity, and can choose different column configurations as needed.
This document evaluates the trade-offs between speed, sensitivity, and resolution when using different ZORBAX column configurations (RR and RRHT) to separate a mixture of nitrogen-containing cardiac drugs. It demonstrates how high-performance liquid chromatography (HPLC) methods can be optimized by selecting specific column dimensions and particle sizes.
| Column Type | ZORBAX Rapid Resolution (RR) and Rapid Resolution High Throughput (RRHT) |
|---|---|
| Stationary Phase | ZORBAX StableBond-C18 |
| Flow Rate | 2 mL/min |
| Operating Temperature | 60 C |
| Detector | Diode Array Detector (DAD) at 230 nm |
The 4.6 x 250 mm, 3.5 um RR column is the best choice if resolution is the overriding factor.
The 4.6 x 50 mm RRHT column is the best choice when high throughput is the primary factor.