This article introduces how to use Agilent 1200 series LC system for impurity analysis, and gives the structure elucidation method of impurities
The document describes a method for identifying minor impurities and by-products in pharmaceutical drug development. It highlights the importance of using high-confidence techniques to detect potential toxic effects associated with these compounds during manufacturing processes.
The note details an experimental setup combining Agilent 1200 Series Rapid Resolution LC systems with Agilent 6000 Series mass spectrometers (Ion Trap and TOF). The study utilizes specific chromatographic gradients and mass spectrometric capabilities, including MS/MS fragmentation and accurate mass measurement, to elucidate the structures of detected impurities.
The measured mass for Impurity D is m/z 250.1804.