This Application Note describes the performance of the system using a 7-peptide sample and a tryptic digest of four different proteins.
This document details the performance of the Agilent 1100 Series microfraction collection/spotting system as a tool for protein identification using LC MALDI. The system serves as a link between separation and detection, dispensing accurate volumes from 100 nL to a few pL to avoid tedious manual spotting procedures.
| Dispensing volume range | 100 nL to a few pL |
|---|---|
| Spotting rate | two spots per minute |
| Matrix flow rate | 1 pL/min |
| MS data accumulation | 400 laser shots per spot |
It allows for accurate dispensing of small volumes to avoid tedious manual spotting procedures with low reproducibility.
Matrix addition can be achieved using the Agilent matrix addition kit with an external syringe pump delivering the solution via a low dead volume T-piece.