Using an Agilent 1200 Series LC system, featuring the G1316B Thermostatted Column Compartment, is used to generate reproducible chromatography with a programmable tem- perature gradient
This document presents an application study demonstrating the reproducibility of temperature gradient chromatography using the Agilent 1200 Series Rapid Resolution Liquid Chromatography System. The study utilizes the G1316B Thermostatted Column Compartment to program column temperature over time, aiming to improve chromatographic efficiency and promote green chemistry by reducing dependence on organic solvents.
The experiment analyzes a mixture of four sulfa drugs using two different organic solvents: methanol and acetonitrile. Results show that retention time and peak area reproducibility are highly consistent, with relative standard deviations (%RSD) of less than 0.070% for retention time and less than 0.269% for peak area. The document details the specific LC conditions, including the use of a ZORBAX SB-C18 column and specific gradient programs for both solvents.
| Column | Agilent ZORBAX SB-C18, 4.6 mm x 250 mm, 5 um |
|---|---|
| Injection volume | 5 uL |
| Retention time reproducibility (Acetonitrile) | < 0.070 %RSD |
The retention time reproducibility is less than 0.070 %RSD.
The study uses an Agilent ZORBAX SB-C18 column with dimensions 4.6 mm x 250 mm and 5 um particle size.