This note describes the effect of coeluting internal standard concentration on the dynamic range of Ion Trap quantitative analyses and demonstrates the advantages of Ion Trap technology over triple-quadrupole instruments for quantitative applications in high-throughput environments.
This document is an application note by Agilent Technologies demonstrating the performance of the Agilent 1100 Series LC/MSD Trap ion trap mass spectrometer. It specifically addresses the effect of coeluting internal standards on quantitative analyses, showing that modern ion trap designs maintain linearity over three orders of magnitude even with vastly excessive concentrations of internal standards.
| Mobile phase A | 1% Acetic acid (Aq) |
|---|---|
| Gradient | Isocratic at 15% B |
The LC/MSD Trap demonstrates linearity over three orders of magnitude for quantitative assays.
No, the presence of increasing amounts of coeluting internal standard has no effect on analytical linearity or instrument performance.
Full scan MS/MS mode provides structural information for reliable compound identification and allows quantitation ions to be selected post-acquisition.