The Dynamic MRM (DMRM) algorithm in the Agilent 6400 Series Triple Quadrupole LC/MS system automatically constructs DMRM timetables for multiple analytes throughout the LC/MS analysis based on the retention time window for each analyte. It allows the instrument to acquire MRM data only during a stated retention time window, thus reducing the number of concurrent ion transitions. In this study, 194 MRM transitions from a digest of complex protein standard was analyzed using DMRM and MRM modes in the Agilent 6410 Triple Quadrupole LC/MS system. Improved sensitivity, better peak symmetry, and up to ten-fold increase in the signal-to-noise (S/N) ratio are achieved using DMRM.
The document describes the Dynamic MRM (DMRM) algorithm in the Agilent 6400 Series Triple Quadrupole LC/MS system, which automatically constructs timetables for multiple analytes based on retention time windows. This approach reduces the number of concurrent ion transitions, allowing the instrument to acquire MRM data only when compounds are expected to elute.
A study was conducted using the Agilent 6410 Triple Quadrupole LC/MS system to analyze 194 MRM transitions from a complex protein standard. The results showed that DMRM provides improved sensitivity, better peak symmetry, and up to a ten-fold increase in the signal-to-noise (S/N) ratio compared to standard MRM mode.
| System Model | Agilent 6400 Series Triple Quadrupole LC/MS system |
|---|---|
| Number of transitions analyzed | 194 MRM transitions |
| Dwell time (MRM mode) | 5 ms |
| Cycle time (MRM mode) | 1650 ms |
| Minimum dwell time (DMRM mode) | 53.4 ms |
DMRM reduces the number of concurrent ion transitions by acquiring MRM data only during stated retention time windows, which improves sensitivity and peak symmetry.
The study observed up to a ten-fold increase in the signal-to-noise (S/N) ratio when using DMRM compared to standard MRM.
The study utilized the Agilent 6410 Triple Quadrupole LC/MS system coupled to an HPLC-Chip/MS system.