Agilent 6460 Series Triple Quadrupole LC/MS/MS Multi-Residue Pesticide Analysis Application Note

About this manual

The document details the analysis of pesticide residues in food and environmental samples using Liquid Chromatography/Mass Spectrometry/Mass Spectrometry (LC/MS/MS) with a triple quadrupole instrument. It highlights the challenges of monitoring hundreds of analytes and introduces Dynamic Multiple Reaction Monitoring (MRM) as a technique to improve sensitivity and specificity compared to time-segmented methods. The note demonstrates how to set up methods quickly using the Agilent Pesticides Application Kit, which includes a 600-compound pesticide MRM database and pre-tested methods.

What's inside

• Introduction
• Database Browser
• Experimental
• Spinach Sample Preparation
• Results and Discussion
• Positive and Negative Ion Test Mix
• Dynamic MRM Method Creation

Specifications

Frequently asked questions

What is Dynamic Multiple Reaction Monitoring (MRM)?

Dynamic MRM is a technique where analyte ions are monitored only while they are eluting from the LC, significantly improving MS duty cycle time for complex samples.

What components are included in the Agilent Pesticides Application Kit?

The kit contains a pesticide test mix, a 600-compound pesticide MRM database, a quick start guide, and several dynamic MRM methods.

Which instruments were used to demonstrate the methods?

The methods were demonstrated using an Agilent 1200 SL Series Rapid Resolution LC and the Agilent 6460 Series Triple Quadrupole LC/MS System.