This application note describes a method for high-resolution analysis of intact triglycerides under non-aqueous reversed phase gradient conditions using the Agilent 1290 Infinity LC System.
The document details a method for analyzing intact triglycerides from vegetable and animal sources using reversed-phase HPLC under non-aqueous conditions. The study focuses on maximizing resolution for soybean oil, which is significant for biofuel production.
The analysis utilizes the Agilent 1290 Infinity LC System equipped with ultraviolet/visible (UV/VIS) Diode Array detection. Key experimental parameters include the use of C18 columns with high pressure configurations (up to 1200 bar), mobile phases consisting of acetonitrile mixed with isopropanol or methyl tert-butyl ether, and specific gradient elution profiles.
The system is capable of operating at pressures up to 1200 bar, which is approximately 18,000 psi.
The column was prepared for operation at a temperature of 20 °C.
The maximum observed pressure reached 760 bar.