The method is used for the simultaneous determination of four β2-agonist residues of terbutaline, salbutamol, clenbuterol and formoterol in pork. The method uses Agilent SampliQ SCX solid-phase extraction (SPE) cartridges and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to measure the residues of four β2-agonists in pork. The method provides a sub-ng/g level of limit of detection (LOD) for all four β2-agonists in pork. The dynamic calibration ranges for these compounds are obtained from 0.25 to 5 ng/g. The overall recoveries range from 78% to 101% with RSD values between 1.8 and 7.2%.
This document details an analytical method developed by Agilent Technologies for the simultaneous determination of four beta-2 agonist residues (terbutaline, salbutamol, clenbuterol, and formoterol) in pork. The study validates a procedure that combines liquid-liquid extraction with solid-phase extraction using Agilent SampliQ SCX cartridges to purify samples before quantification via Liquid Chromatography-Tandem Mass Spectrometry (LC-ESI-MS/MS).
The application note provides specific experimental conditions, including reagent preparation, sample hydrolysis protocols, and instrument parameters for the Agilent 1200 HPLC system and Agilent 6460 Triple Quadrupole LC-MS/MS. It reports validation results such as limits of detection (LOD), linearity, recovery rates ranging from 78% to 101%, and reproducibility data for the target compounds in pork matrices.
| Cartridge Part Number | 5982-3236 |
|---|---|
| Column Part Number | 959741-906 |
| Manifold Part Number | 5982-9120 |
| Flow Rate | 0.4 mL/min |
| Column Temperature | 40°C |
The method determines terbutaline, salbutamol, clenbuterol, and formoterol.
The LOD for clenbuterol is 0.02 ng/g.
The overall recoveries range from 78 to 101%.