This application note describes a rapid method for screening and confirmation of melamine, ammelide, ammeline and cyanuric acid in infant formula and soy meal using the Agilent 7890A/7000A Series Triple Quadrupole GC/MS and backflushing with a Purged Ultimate Union. The extraction and derivatization procedures are the same as those used in the FDA GC/MS method. Excellent linearity (R2 > 0.99) was obtained in the range of 0.16 to 2.5 ppm, with run times less than 15 minutes.
The document details a modification of the FDA GC-MS method adapted for the Agilent 7890A gas chromatograph equipped with an Agilent 7000A Series Triple Quadrupole GC/MS. The method utilizes a purged union configuration and backflushing to achieve run times under 15 minutes while detecting melamine, ammelide, ammeline, and cyanuric acid at 0.25 ppm.
| Detection Limit | 0.25 ppm for melamine and its analogs |
|---|---|
| Linearity Range | 0.16 to 2.5 ppm |
| Run Time | Less than 15 minutes |
| Column Configuration | Two 15 m x 0.25 mm x 0.25 pm HP-5ms columns |
| Inlet Temperature | 280 °C |
The method detects melamine, ammelide, ammeline, and cyanuric acid.
It provides a sensitivity of 0.25 ppm, meeting the FDA import alert requirement which is lower than the previous 2.5 ppm limit.