This application note presents the capability of the Agilent Time of Flight Mass Spectrometer (LC/MSD TOF) to be used as a screening, confirmation and quantitation tool-within-one analytical run of 5 min.
This document presents the capability of the Agilent Time of Flight Mass Spectrometer (LC/MSD TOF) to screen for and quantify Sudan Red I, II, III, and IV in food matrices within a 5-minute analytical run. The text details the regulatory background regarding the ban on these carcinogenic dyes and the necessity for rapid detection in the food industry.
The note provides specific experimental conditions for the Agilent 1100 Series LC/MSD TOF system, including LC parameters using a ZORBAX XDB C18 column and MS settings with ESI positive ionization. It demonstrates how accurate mass determination (<3 ppm) and extracted ion chromatogram (EIC) techniques enhance the confidence in identifying these banned dyes in complex samples like chili powder and spice oil.
| Mass Accuracy | <3 ppm |
|---|---|
| Analysis Time | 5 min |
| Column | ZORBAX XDB C18, 2.1 mm x 50 mm, 1.8 um |
| Flow Rate | 0.5 mL/min |
| Gas Temp | 350 °C |
| Drying Gas | 8 L/min |
| Nebulizer Pressure | 46 psi |
| Capillary Voltage | 4000V |
The analytical run for screening, confirmation, and quantitation of Sudan Reds is 5 minutes.
The document covers Sudan Red I, II, III, and IV.
The TOF mass spectrometer provides accurate mass determination with less than 3 ppm error.