This paper introduces a rapid LC/MS method for determining hydroxymethylfurfural in foods. The method has high sensitivity, selectivity, and a low detection limit
This document presents a sensitive and selective liquid chromatography-mass spectrometry (LC/MS) method for monitoring hydroxymethylfurfural (HMF) in food products. HMF is an indicator of food deterioration, and this method addresses toxicological concerns by providing a reliable way to detect it at low concentrations.
The procedure involves aqueous extraction of the sample, cleanup using solid-phase extraction (SPE) with an Oasis HLB cartridge, and analysis using an Agilent 1100 series HPLC system coupled with an Agilent 1100 MS detector. The method utilizes positive APCI ionization and selected ion monitoring (SIM) to achieve a detection limit of 0.005 µg/g.
| Column | ZORBAX Bonus RP Narrow Bore |
|---|---|
| Flow Rate | 0.2 mL/min |
| Ionization Mode | Positive APCI |
| Mobile Phase | 0.01 mM acetic acid in 0.2% aqueous solution of formic acid |
The separation is performed on a ZORBAX Bonus RP Narrow Bore column.
The flow rate is set to 0.2 mL/min.