An improved analytical method that offers rapid, accurate determination and identification of 22 amino acids in a variety of matrixes is reported. The amino acids were extracted from the matrixes using acidified water. Simul- taneous determination of 22 underivatized amino acids was carried out by liquid chromatography–mass spec- trometry (LC/MS). A narrow-bore column allowed rapid screening and quantitative analysis by LC/atmospheric pressure chemical ionization (APCI) MS in positive ion mode.
The document presents a study by Hacettepe University and the Scientific and Technical Research Council of Turkey detailing an improved analytical method for the rapid, accurate, and repeatable determination of 22 free amino acids. The method utilizes acidified water extraction followed by simultaneous analysis via LC/APCI-MS in positive ion mode.
| Column | ZORBAX Bonus-RP, narrow-bore (100 mm x 2.1 mm, 3.5 um) |
|---|---|
| Flow rate | 0.2 mL/min |
| Mobile phase | 0.01 mM acetic acid in 0.2% aqueous solution of formic acid |
| Injection volume | 20 uL out of 1000 pL |
| Ionization mode | Positive APCI |
| Dwell time | 5oV 27 ms |
The method allows for the simultaneous determination of 22 underivatized amino acids.
The SIM-MS analysis showed excellent identification and quantification conditions within a 7.5 min total run time.
The study analyzed baby foods, vegetables, fruits, juices, nuts, wine, beer, milk, chicken, and honey.