The analytical method uses LC/MS/MS to detect 4 banned nitrofuran antibiotics metabolites, furazolidone, furaltadone, nitrofurantoin and nitrofurazone in fish, and the method is highly sensitive, down to 0.01 ppb (10 ppt). The method meets all the confirmation criteria set by the European Union for analytical method performance. The method is suitable for the analysis of fish and other aquaculture samples.
This document presents an application for the analysis of nitrofuran antibiotic metabolites in tilapia samples using LC/MS/MS. The method utilizes the Agilent 6410 triple quadrupole mass spectrometer to detect four specific metabolites: aminohydantoin (AH), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 3-amino-2-oxazolidinone (AOZ), and semicarbazide (SC). The study demonstrates the system's high sensitivity, achieving detection limits of 0.01 ppb (10 ppt) for each analyte.
| Detection Limit | 0.01 ppb (10 ppt) |
|---|---|
| Calibration Range | 0.1 ppb to 10 ppb |
| Minimum Required Performance Level | 1 g/kg |
| Column Temperature | 40°C |
| Mobile Phase A | 0.1% formic acid in water |
| Mobile Phase B | acetonitrile |
| Flow Rate | 0.3 mL/min |
| Drying Gas Flow | 10 L/min |
| Nebulizer Pressure | 35 psig |
| Drying Gas Temperature | 350°C |
The method is shown to be highly sensitive, to 0.01 ppb (10 ppt), for each of the four analytes.
The four compounds analyzed are furazolidone, furaltadone, nitrofurantoin, and nitrofurazone metabolites (specifically AH, AMOZ, AOZ, and SC).
Quantitative results were performed with the new MassHunter quantitative analysis software.