The use of liquid chromatography/ion trap mass spectrometry (LC/ITMS) and liquid chromatography/time-of-flight mass spectrometry (LC/TOFMS) for the identification and quantitation of ter- buthylazine in olive oil samples
The document details a method for analyzing pesticide residues, specifically terbuthylazine, in complex food matrices like olive oil. The procedure involves sample preparation via liquid-liquid extraction followed by matrix solid-phase dispersion (MSPD) using aminopropyl-bonded silica and Florisil clean-up.
Analysis is performed using two Agilent 1100 Series mass spectrometry configurations: LC/ITMS in MS/MS mode monitoring the fragment ion at m/z 174, and LC/TOFMS utilizing accurate mass measurements of the protonated molecule [M+H]+ at m/z 230 and characteristic chlorine isotope clusters.
The method uses a preliminary liquid-liquid extraction followed by matrix solid-phase dispersion (MSPD) using aminopropyl-bonded silica as a sorbent.
The accuracy typically obtained was routinely better than 2 ppm.