This document describes a rapid method for the analysis of paraben preservatives using reversed-phase HPLC.
This document details a method for the separation and analysis of paraben preservatives (methyl, ethyl, and propyl) and 2-phenoxyethanol (2PX) in foods, beverages, and cosmetics. The authors, Coral Barbas, Javier Rupérez, Andre Dams, and Ronald E. Majors, present a technique using reversed-phase HPLC to separate these compounds effectively.
The method utilizes a ZORBAX Eclipse XDB-C18 Rapid Resolution column with a mobile phase consisting of water and methanol. The analysis is performed at a flow rate of 0.8 mL/min and a temperature of 40°C, with detection at 254 nm. The document includes chemical characteristics, chromatographic conditions, and results demonstrating baseline separation within 16 minutes.
| Mobile phase A | Water |
|---|---|
| Mobile phase B | Methanol |
| Flow rate | 0.8 mL/min |
| Temperature | 40°C |
| Detector | UV 254 nm |
The method uses a ZORBAX Eclipse XDB-C18 Rapid Resolution column.
The analysis is completed in 16 minutes.