This article introduces the analysis method of fatty acid methyl esters (FAMEs), including the separation using different stationary phases. It is found that polyethylene glycol columns are suitable for the separation of simple samples, while medium polar cyanopropyl columns (DB23) are suitable for the separation of complex FAME mixtures and achieve some cis-trans separation. The highly polar HP-88 cyanopropyl column is preferred for more detailed cis-trans separation.
The document details the analysis of fatty acid methyl esters (FAMEs) derived from food. It evaluates three different column types: polyethylene glycol columns (DB-Wax), medium polar cyanopropyl columns (DB-23), and highly polar HP-88 columns.
Standards are typically dissolved in hexane at a concentration between 0.01% and 0.1% (w/v).
The analysis uses a 30 m x 0.25 mm ID, 0.25 µm DB-Wax column.