This article introduces a method for studying DNA sequence copy number alterations, which uses a new labeling technology to extract DNA from formalin-fixed, paraffin-embedded (FFPE) tissues and obtain high-quality data.
This document details a protocol for performing Copy Number Analysis on archival Formalin-Fixed Paraffin-Embedded (FFPE) tumor samples using Agilent's Oligo Array CGH technology. It addresses the challenges of analyzing degraded DNA from FFPE tissues, which often yield noisy data due to fragmentation and damage. The note introduces a non-enzymatic labeling method based on Kreatech's Universal Linkage System (ULS) technology.
| Average Spatial Resolution | ~35 kb |
|---|---|
| Labeling Time | 30 minutes |
| Hybridization Time | 40 hours |
| Product Part Number | 5190-0419 |
The protocol uses Kreatech's Universal Linkage System (ULS) technology, a non-enzymatic direct labeling methodology.
The non-enzymatic ULS technology is four times faster, contains fewer steps, is easier to automate, and is independent of fragment length.
Yes, the method clearly detected the frequent amplification of the HER-2 oncogene in DNA isolated from 1-, 4-, and 10-year-old breast tumor FFPE blocks.