This article describes the determination of aflatoxins using Agilent 1290 Infinity LC, Agilent 1260 Infinity Binary LC, and Agilent ZORBAX RRHT 1.8 µm columns by fluorescence detection after electrochemical derivatization. The use of shorter columns instead of conventional 5 µm columns accelerates the HPLC and post-column derivatization method for the determination of aflatoxins B1, B2, G1, and G2. Despite the use of the Coring Cell and related band broadening techniques, high resolution was achieved. The analysis time was approximately 10 minutes, including column flushing, and the sample throughput was increased by more than 2 times.
The document details a method for determining aflatoxins B1, B2, G1, and G2 in food samples such as hazelnuts and marzipan. It utilizes Agilent 1290 Infinity LC or 1260 Binary LC systems coupled with fluorescence detection following electrochemical derivatization using a Coring Cell.
The primary focus is demonstrating that high-resolution analysis can be achieved rapidly by transferring methods from conventional columns to shorter, high-efficiency ZORBAX RRHT 1.8 µm columns. The text confirms that this approach reduces analysis time by more than half (to approximately 10 minutes including flushing) while maintaining comparable resolution and sensitivity.
The analysis takes about 10 minutes, which includes the time required for flushing the column.
All aflatoxins are separated within a reduced analysis time of 8.0 minutes.
Post-column derivatization requires a four second reaction time between the Coring Cell and detector.