This document introduces the Cary 4000/5000/6000i series instruments measuring 0.8ul small volume samples functions and characteristics, and demonstrates the advantages of the series instruments in small volume, high absorption samples measurement through the study of protein light harvesting complexes photobleaching phenomenon.
This document describes a study on the photodegradation of bacterial porphyrins and other protein light-harvesting complexes. It highlights how the Microvolume capability of High-performance UV/Vis/NIR spectrophotometers allows for investigations into samples that are too concentrated or available only in very small amounts.
The system can measure samples with volumes as small as 0.8ul.
Noise levels as low as 0.0002 Abs can be achieved with samples of 1ul.
The dynamic range is about 7 Abs in the TrayCell.