Agilent Identification Characterization of a Novel Delivery Molecule for a Clandestine PDE-5 Inhibitor Drug in a Dietary Supplement Application Note

About this manual

This document details a forensic analysis performed by Flora Research Laboratories to detect synthetic PDE-5 inhibitors in herbal dietary supplements. The study focuses on a sample containing a novel prodrug analogue of aildenafil, a known PDE-5 inhibitor.

The analysis utilized the Varian 500-MS ion trap LC/MS/MS system with the TurboDDS feature to screen for unknown compounds. Subsequent characterization using high-resolution FT-MS and NMR spectroscopy confirmed the presence of the aildenafil-like core structure and determined the empirical formula of the prodrug.

What's inside

• Introduction
• Results and Discussion
• TurboDDS Analysis
• FT-MS Data
• NMR Data
• Conclusion
• References

Frequently asked questions

What is the Varian 500-MS used for in this study?

It was used to screen a dietary supplement sample for unknown synthetic PDE-5 inhibitors using TurboDDS technology.

How does the TurboDDS feature on the 500-MS work?

It performs a survey scan and automatically triggers MS experiments if an unknown contaminant is above a given instrument threshold.

What exact mass was measured for the prodrug compound?

The FT-MS measurement indicated an exact mass of 630.22820.