Varian 500-MS Ion Trap Mass Spectrometer Application Note 02101

This application note describes how to analyze bumetanide in a dietary supplement using the Varian 500-MS Ion Trap Mass Spectrometer in positive and negative ionization mode.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 111 KB
Published: 12 June, 2012
Updated: 30 September, 2023
Model: 500-MS
MD5 checksum: 4505EAA864D601FC968B1C866D633F18
PDF: Varian 500-MS Ion Trap Mass Spectrometer Application Note 02101 PDF

About this manual

This document details an application for analyzing the banned substance bumetanide in dietary supplements using the Varian 500-MS LC/MS/MS Ion Trap. The method utilizes electrospray ionization (ESI) in both positive and negative modes to detect and confirm the presence of the drug.

What's inside

Introduction
Sample Preparation
HPLC Conditions
Instrumentation
MS Parameters
Results and Discussion
Conclusion

Specifications

Column Temp	30°C
Drying Gas	30 psi at 350 °C
Calibration Range	1 to 20 pg/mL

Frequently asked questions

What instrument is used for the analysis?

The Varian 500-MS LC/MS/MS Ion Trap is used for the analysis.

Can the analysis be performed in both positive and negative modes?

Yes, the analysis is performed in positive and negative mode simultaneously.

What is the detection limit range for the calibration curve?

The calibration curve covers a range from 1 to 20 pg/mL.

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