This application note describes the use of the Varian 320-MS Triple Quadrupole LC/MS/MS for the analysis of 15 azo dyes. This method provides high sensitivity, accuracy and selectivity, making it a useful tool for regulatory monitoring and quality assessment.
Azo compounds are widely used in textiles, food, and paper industries but can pose health risks. This document outlines an analytical method using LC/MS/MS to detect and quantify these compounds with high sensitivity and selectivity.
The note details the instrumentation setup, including the Varian 320-MS Triple Quadrupole Mass Spectrometer with ESI source. It provides specific HPLC conditions, API parameters, and MS/MS transition data for analyzing 15 different azo dyes.
The method allows detection and quantification from 5 ppb to 200 ppb.
The specified injection volume for the analysis is 20 µL.