This application note describes a method for the analysis of perchlorate using the Varian 1200L triple quadrupole mass spectrometer. The method has high selectivity and sensitivity and can be used to analyze perchlorate in contaminated water sources.
The document presents a method for analyzing perchlorate, a water-soluble anion found in munitions, rocket propellants, and fertilizers, which poses environmental and health risks. The method utilizes the Varian 1200L triple quadrupole mass spectrometer equipped with an ESI source and Varian ProStar 210 binary solvent delivery modules. The analysis involves filtering and treating water and produce samples to remove free chlorine and sulfate before homogenization and sonication.
Experimental conditions include the use of a Dionex AS-16 column with a mobile phase of 65mM KOH and Methanol. The method operates in negative ionization mode with Multiple Reaction Monitoring (MRM) transitions monitored simultaneously for quantitation. Results demonstrate a linear calibration curve from 0.2 to 5 ppb and a detection limit of 14 ppt for perchlorate in various matrices, including tap water, celery, zucchini, green beans, and date sugar.
| Mobile Phase A | 65mM KOH |
|---|---|
| Mobile Phase B | Methanol |
| Flow Rate | 300 pL/min |
| Mobile Phase Ratio | 60:40 A:B |
| Suppressor Regenerant | 72mM HpSO4 |
| Calibration Range | 0.2 to 5 ppb |
| Correlation Coefficient | r=0.9998 |
| Detection Limit | 14 ppt |
The detection limit for perchlorate analysis was calculated to be 14 ppt.
The calibration curve for quantitation of perchlorate is linear from 0.2 to 5 ppb.