This document describes a method for the identification and quantification of pesticides and herbicides in water using liquid chromatography (LC) and diode array detector (DAD). The method was developed not only for the separation and detection of 17 common herbicides but also 19 additional herbicides and pesticides of possible interest. The methodology consists of: • Sample preparation by Solid Phase Extraction using Empore Disk technology • Separation by HPLC • Peak identification by Spectral Library Search using a Diode Array Detector
The document outlines a methodology developed to determine herbicides in water, complying with regulations effective in France and other European countries. The method utilizes Solid Phase Extraction (SPE) using Empore disk technology for sample preparation, followed by separation via High-Performance Liquid Chromatography (HPLC). Peak identification is achieved through spectral library search using a Varian Polychrom Diode Array Detector.
The procedure requires applying exactly 1 liter of water sample.
The complete separation and identification of all 36 compounds takes 105 minutes.
These specific compounds are detected at a selective wavelength of 244 nm.