This application note describes a method for rapid separation and quantitation of a mixture of 10 non-steroidal anti-inflammatory drugs (NSAIDs) using fast high-performance liquid chromatography (HPLC) and diode array detector (DAD). Separations are performed in less than 5 minutes using a 3-cm long column. To enhance resolution, two wavelengths, 254 and 360 nm, are used. Quantitation is performed by integrating the peak areas of the absorbance peaks at each wavelength. This method can be used to rapidly identify and quantify the NSAIDs present in a mixture.
This document details a method for analyzing mixtures of 10 non-steroidal anti-inflammatory drugs (NSAIDs) using fast HPLC with diode array detection. The primary challenge addressed is the co-elution of components, specifically ibuprofen, oxaprofen, and indomethacin, which overlap in standard chromatographic runs.
The text explains how Varian's PolyView multicomponent analysis (MCA) software utilizes spectral data to identify and accurately quantitate these co-eluting compounds. By analyzing the differences in individual spectra within the fused peaks, the software can resolve components that would otherwise be indistinguishable at a single wavelength, allowing for accurate quantitation without requiring longer, slower columns.
The software can identify and quantitate up to 6 coeluting compounds.
The mixtures of 10 non-steroidal anti-inflammatory drugs were analyzed in less than 4 minutes.
The analysis was performed over the 220-325 nm wavelength range.