This application note describes how to use Agilent PLgel MIXED-B columns to analyze reacetylated polyvinyl alcohol (PVAc).
Polyvinyl alcohols (PVOH) are water-soluble polymers that can be analyzed by aqueous size exclusion chromatography. However, when they are reacetylated to form polyvinyl acetate (PVAc), the material becomes soluble in tetrahydrofuran (THF). In this scenario, Agilent PLgel MIXED-B columns are recommended as the optimal choice for analysis.
The document details a specific method using two PLgel 10 µm MIXED-B columns with dimensions of 300 x 7.5 mm. The eluent used is THF at a flow rate of 1.0 mL/min, with an injection volume of 100 µL containing 0.25% loading and detection via RI (Refractive Index).
| Column Dimensions | 300 x 7.5 mm |
|---|---|
| Eluent | THF |
| Flow Rate | 1.0 mL/min |
| Loading | 0.25%, 100 wL |
| Detection Method | RI |
Agilent PLgel MIXED-B columns are the choice because the resultant polyvinyl acetate is rendered THF soluble.
The analysis uses THF as the eluent.