This document describes the extraction and analysis method of marijuana and its metabolites in human blood, as well as the use of related instruments and reagents.
This document details an effective Solid Phase Extraction (SPE) method coupled with Gas Chromatography/Mass Spectrometry (GC/MS) for the extraction of tetrahydrocannabinol (THC), 11-hydroxy-THC, and THC-COOH from human whole blood. The procedure is designed to detect recent drug usage and impairment.
The sample must be centrifuged at a minimum of 2500 rpm for 5 minutes.
Elution fractions must be evaporated under nitrogen at no higher than 40°C.