A rapid method was developed on the Agilent 7000 Series Triple Quadrupole GC/MS system to screen for more than 150 doping agents in seven classes of substances, at or below WADA MRPLs
This document details a rapid screening method developed on the Agilent 7000A Triple Quadrupole GC/MS system for detecting more than 150 doping agents in urine samples. The method utilizes a short capillary column, rapid scan speed, and hydrogen as a carrier gas to achieve a run time of less than 8 minutes, meeting or exceeding World Anti-Doping Agency (WADA) Minimum Required Performance Levels (MRPLs).
The application note covers the experimental setup, including sample preparation involving glucuronidase incubation and liquid-liquid extraction, followed by derivatization with MSTFA. It provides specific gas chromatography and mass spectrometry conditions, such as the use of an Agilent J&W HP-1 Ultra Inert column and Multiple Reaction Monitoring (MRM) acquisition parameters. The text also discusses the method's ability to quantify endogenous steroids and qualitatively analyze a wide range of exogenous substances, including anabolic steroids, beta-agonists, and narcotics.
| Run Time | less than 8 minutes |
|---|---|
| Number of Doping Agents Screened | more than 150 |
| Carrier Gas | Hydrogen |
| Column Length | 12.5 m |
| Injection Volume | 5 pL |
| Source Temperature | 280 °C |
| Collision Gas Flow | 1.5 mL/min |
The method achieves a run time of less than 8 minutes.
The system is capable of screening for more than 150 doping agents.
Hydrogen is used as the carrier gas at a constant flow of 1.0 mL/min.