This Application Note demonstrates the high resolution separation of a pharmaceuti- cal test mixture, a pesticide standard and a cosmetic sample on a 1 m column (4 × 25 cm) using the Agilent 1260 Infinity Analytical SFC system.
This document describes a method for achieving high resolution in Supercritical Fluid Chromatography (SFC) by serially coupling four 25 cm columns to create an effective 1 meter length. This approach allows for the use of small particle sizes at relatively low pressures, significantly increasing plate numbers and peak capacity compared to single column setups.
The Agilent 1260 Infinity Analytical SFC system.
Four serially coupled Agilent ZORBAX Rx-SIL 25 cm x 4.6 mm, 5 µm columns.