This document presents the preliminary results of a collection on an unusual macromolecular RNA G-quadruplex structure. The single crystal data was collected to 2.2Å resolution, providing us with a detailed view of the RNA’s sugar pucker and hydration structure. The efficient data collection geometry and associated high redundancy of data has enabled us to also visualize the anomalous scattering from the ions in the central channel, and confirm the location of the bromine atoms included to assist in phasing.
The document presents preliminary results from collecting single-crystal data on an unusual macromolecular RNA G-quadruplex using an in-house Agilent Technologies (formerly Oxford Diffraction) Nova X-ray source with a Titan CCD detector. The system was used to collect data to 2.2 Å resolution, providing detailed views of the RNA sugar pucker and hydration structure.
The maximum exposure time was 5 minutes per frame.
The total collection time was 22 hours.
The single crystal data was collected to 2.2A resolution.