This note shows how the Agilent PLgel 5 µm gel permeation chromatography column matches the performance of another leading column, even though it has slightly different dimensions.
This document addresses the concern that using a chromatography column with different dimensions might disrupt a standard method. It presents data showing that the Agilent PLgel 5 µm column matches the performance of a leading competitor column despite having slightly different dimensions.
The note details a comparison using polycarbonate as a test analyte. Results from overlaid chromatograms and molecular weight averages show that the difference in elution time is negligible once calibrated, allowing analysts to interchange 7.5 mm columns for 7.7 mm columns without changing the method.
| Eluent | THF |
|---|---|
| Flow Rate | 1.0 mL/min |
| Temperature | Room temperature |
| Detector | RI |
| System | PL-GPC 50 |
Yes, analysts can interchange 7.5 mm columns for 7.7 mm columns without changing the method as the results will be indistinguishable.
Polycarbonate was used as the test analyte to demonstrate the indistinguishable performance of the columns.