This Application Note describes how the Agilent 380-ELSD Evaporative Light Scattering Detector was used to analyze herbicides at low temperature. The detector is designed to evaporate highly aqueous mobile phases at ambient temperature, providing a solution for detecting thermally sensitive compounds.
This document details an application note by Stephen Ball from Agilent Technologies regarding the analysis of herbicides using HPLC with low temperature evaporative light scattering detection. It focuses on the Agilent 380-ELSD Evaporative Light Scattering Detector, which is designed to evaporate highly aqueous mobile phases at ambient temperature, solving detection difficulties for thermally unstable phenylurea compounds.
The detector is designed to determine the effect of varying the evaporation temperature during the analysis of a mixture of herbicides with different volatilities.
The nebulizer temperature is set to 25 °C.
Only seven of the ten herbicides were detected at 70 °C.