CHIRALPAK QN-AX and QD-AX are anion exchanger chiral stationary phases providing specific enantioselectivity for acidic compounds. In this Application Note, we demonstrate that the application of these columns can be extended to supercritical fluid chromatography (SFC) for enantiomer separation of acidic compounds. Using the advantages of the advanced technology of the Agilent 1260 Infinity Analytical SFC system, we were able to assess the influence of a series of parameters such as the mobile phase additives and the flow rate to gain insight into the general approaches for method development and optimization.
This document describes a method for separating enantiomers of acidic compounds using the Agilent 1260 Infinity Analytical SFC System. The study utilizes CHIRALPAK QN-AX and QD-AX chiral stationary phases, which are anion exchangers designed for specific enantioselectivity.
The note details the experimental setup, including the specific modules of the Agilent SFC system used, such as the Aurora SFC Fusion A5 module and the binary pump. It investigates the influence of various parameters, including mobile phase additives (formic acid, ammonium formate) and flow rates, to optimize method development and resolution.
| Column dimensions | 4.6 x 100 mm |
|---|---|
| Flow rate | 3.0 mL/min |
| Column temperature | 40 °C |
The Agilent 1260 Infinity Analytical SFC System.
CHIRALPAK QN-AX and QD-AX columns.
The flow rate is conventionally set at 3.0 mL/min.