This application note describes a method for analyzing 20 contract laboratory protocol (CLP) pesticides in olive oil using an Agilent GC-µECD system. A QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) with dispersive solid phase extraction (dSPE) procedure cleaned the sample prior to analysis. A dual µECD and dual capillary GC column approach accomplished simultaneous primary and confirmatory analysis. The primary column, an Agilent J&W DB-35ms 30 m × 0.25 mm × 0.25 µm and a confirmatory column, an Agilent J&W DB-XLB 30 m × 0.25 mm × 0.50 µm effectively resolved all 20 CLP pesticides. An unpurged two-way capillary flow technology splitter divided the flow from a single injection port to the two analytical GC columns. Endosulfan sulfate and endosulfan 1 were present in the olive oil sample.
The document details a method for analyzing pesticide residues in olive oil, addressing safety concerns related to increased production. The procedure utilizes a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation technique followed by dispersive solid phase extraction (dSPE) to clean the sample matrix.
Analysis is performed using an Agilent 7890A gas chromatograph equipped with dual pECD detectors and a capillary flow technology (CFT) splitter. This setup allows for simultaneous primary analysis on an Agilent J&W DB-35ms column and confirmatory analysis on an Agilent J&W DB-XLB column from a single injection, effectively resolving 20 contract laboratory protocol pesticides.
The primary column is 30 m x 0.25 mm x 0.25 µm, and the confirmatory column is 30 m x 0.25 mm x 0.50 µm.
The average limit of detection across both columns using the lowest concentration standard was 0.3 ng/mL.