Agilent J&W DB-35ms Ultra Inert GC Column Application Note for Organophosphorus Pesticides in Apple Matrix

About this manual

The document describes an analytical method for determining low-level organophosphorus (OP) pesticide residues in apple matrix. It utilizes a capillary flow technology (CFT) device installed post-column to split the effluent between a Mass Selective Detector (MSD) and a Flame Photometric Detector (FPD). This configuration allows for full scan, selective ion monitoring (SIM), and phosphorus-specific detection from a single injection.

The method employs a simplified QuEChERS extraction procedure for sample preparation. The analysis is performed using an Agilent 7890 GC system equipped with an Agilent J&W DB-35ms Ultra Inert column, achieving recovery rates greater than 75% and detection limits between 15-25 ng/mL.

What's inside

• Abstract
• Introduction
• Experimental
• Chromatographic Conditions
• Flow Path Supplies
• Reagents and Chemicals
• Sample Preparation

Frequently asked questions

What is the minimum sample weight required for the apple extraction procedure?

The procedure requires weighing 15 grams of apple sample, with a tolerance of plus or minus 0.1 grams.

What are the specific centrifugation speeds and durations used in the sample preparation steps?

The samples are centrifuged at 4000 rpm for 5 minutes initially, and then at 4000 rpm for 3 minutes after the dispersive SPE step.

What is the split ratio used to divide the column effluent between the MSD and FPD detectors?

The effluent is split in a 3:1 ratio, directing three parts to the MSD and one part to the FPD.