This Application Note describes the separation of four proteins by anion exchange chromatography using four different high salt-containing elution buffers (up to 2 M) for linear and step gradients.
This document presents an application note detailing the separation of four proteins using anion exchange chromatography with high-salt elution buffers. The study highlights the system's ability to withstand harsh conditions, such as 2 M salt concentrations, without the corrosion issues typical of stainless steel-based LC systems. The Agilent 1260 Infinity Bio-inert Quaternary LC System features an iron/steel-free design, ensuring metal-free components in the sample flow-path that interact only with ceramics or PEEK.
| System Design | Iron/steel-free design |
|---|---|
| Flow Path Material | Metal-free components |
| Column Dimensions | 4.6 x 250 mm |
| Flow Rate | 0.5 mL/min |
| Temperature | 25 °C |
| Detection Wavelength | 280 nm |
It has an iron/steel-free design that withstands harsh conditions like high salt concentrations without requiring special cleaning procedures.
1 M sodium acetate (CH3COONa) showed the best resolution among the tested salts.
The stability of retention time and resolution was proven over a period of 48 hours.