This application note presents the performance of Agilent Bond Elut Plexa multi-compound analysis system for the detection of six systemic antifungal drugs in human plasma. The system includes Agilent Bond Elut Plexa polymeric sorbent, Agilent Pursuit XRsUltra 2.8 Diphenyl column and Agilent 1260 Infinity LC Triple Quadrupole LC/MS. Agilent Bond Elut Plexa delivered extremely clean samples, which was demonstrated by detection down to pg levels. All compounds showed near 100% recovery and a single digit % RSD. Superb linearity was achieved in the calibration curves with R2 ¡ 0.995 for all compounds.
The document presents a validated analytical method for quantifying multiple antifungal medications, including fluconazole, ketoconazole, itraconazole, linezolid, voriconazole, and bitertanol, in human plasma samples. The study utilizes solid phase extraction (SPE) with Agilent Bond Elut Plexa plates to achieve clean sample preparation and low detection limits.
Calibration samples were prepared starting from a concentration of 0.05 ng/mL.
The analysis was performed with a flow rate of 0.4 mL/min.
The gas temperature was set to 300 °C.