Agilent Bond Elut QuEChERS AOAC Kit Application Note for Pesticide Residue Analysis

About this manual

This document details a validated method for extracting and cleaning up 16 pesticide residues from apple samples. The procedure utilizes the Agilent Bond Elut QuEChERS AOAC Extraction kit (p/n 5982-5755) followed by cleanup with Bond Elut QuEChERS AOAC dispersive SPE kits (p/n 5982-5022 and 5982-5058). The prepared extracts are analyzed using an Agilent 1200 HPLC coupled to a 6410 Triple Quadrupole LC/MS/MS system.

What's inside

• Abstract
• Introduction
• Reagents and Chemicals
• Solutions and Standards
• Equipment and Material
• Instrument Condition
• Sample preparation

Frequently asked questions

What is the limit of quantitation for pesticides in apple using this method?

The limit of quantitation is 5 ng/g.

What spiking levels were used for the recovery experiments?

The spiking levels were 10, 50, and 200 ng/g.

What is the average mean recovery percentage for the pesticides tested?

The average mean recovery is 95.4%.