This application note describes the development of a sample extraction, cleanup, and enrichment method with an Agilent Bond Elut Plexa cartridge and a separation method using an Agilent Poroshell 120 column, with quantification by HPLC. The method is simple and effective for the extraction, enrichment, and analysis of multiple phenol compounds in drinking water.
This document details a method for the simultaneous determination of 11 phenols in drinking water. The procedure utilizes solid phase extraction (SPE) with an Agilent Bond Elut Plexa cartridge for sample extraction, enrichment, and cleanup. Separation is achieved using an Agilent Poroshell 120 column with HPLC.
| Solid phase extraction cartridge | Agilent Bond Elut Plexa cartridge, 80 mg, 3 mL |
|---|---|
| Vacuum manifold | Agilent Vac Elut 20 Manifold Tall Glass Basin |
| Mobile phase A | 0.1% Formic acid in water |
| Mobile phase B | 0.1% Formic acid in acetonitrile |
| Flow rate | 0.4 mL/min |
| Detection | UV, 280 nm |
| Sample volume | 250 mL |
The method analyzes 11 phenol compounds including Phenol, 4-nitrophenol, 2-chlorophenol, and Pentachlorophenol.
Overall recoveries ranged from 87% to 108%.
The RSD values were between 1.4% and 6.7%.