This paper presents an analytical method for the determination of 11 quinolone antibiotic residues in bovine liver. The method involves a rapid and efficient pretreatment using Agilent Bond Elut QuEChERS kits, followed by extraction and partitioning, and dispersive solid phase extraction for cleanup. The compounds are then determined using LC-ESI-MS-MS in positive ion MRM mode. The method demonstrates good accuracy and precision, with a limit of quantification significantly lower than the maximum residue limit for quinolones in food producing animals.
The document presents an analytical method to determine 11 specific quinolone antibiotics (pipemidic acid, ofloxacin, ciprofloxacin, danofloxacin, lomefloxacin, enrofloxacin, sarafloxacin, cinoxacin, oxolinic acid, nalidixic acid, and flumequine) in bovine liver samples. The procedure utilizes Agilent Bond Elut QUEChERS kits for rapid pretreatment involving extraction, partitioning with salts, and dispersive solid phase extraction (dispersive-SPE) cleanup.
Norfloxacin was selected as the internal standard.
The established limit of quantification was 5 ng/g.
The total cycle time for a single run is approximately 16 minutes.