This application note demonstrates the concordance in the results achieved with identical oligonucleotide probes on two versions of Agilent miRNA microarrays [(AMADID 021827, release 12.0) and (AMADID 031181, release 16.0)], based on different revisions of the miRBase database and provided on different microarray formats. Since probes for a given microarray will remain constant for subsequent versions of Agilent SurePrint G3 Human miRNA microarrays, barring any miRBase sequence changes, it is necessary to confirm that the TotalGeneSignal achieved is consistent. The data show Agilent’s strength in probe reproducibility, a crucial factor for identifying miRNA of differential expression.
This document evaluates the data reproducibility and concordance between two versions of Agilent SurePrint G3 Human miRNA microarrays: AMADID 021827 (Release 12.0) and AMADID 031181 (Release 16.0). The study compares results generated using identical oligonucleotide probes but different revisions of the miRBase database and different microarray formats.
The analysis utilized four Agilent RNA samples, including Universal Human miRNA Reference RNA and MVP Total RNA from Human Placenta, Brain, and HeLa cells. Results indicate tight correlation between technical replicates and high concordance of signal intensities for identical probes across both versions, confirming the reliability of the SurePrint G3 platform.
The microarrays were tested using Universal Human miRNA Reference RNA, MVP Total RNA from Human Placenta, MVP Total RNA from Human Brain, and MVP Total RNA from Human HeLa 3 Cells.
The study compared the SurePrint G3 Human miRNA microarray Release 16.0 (8X60K) with the Release 12.0 version (8X15K).
The correlation plots show an R2 value of 0.99 for the signal intensities between the different AMADID versions.