Agilent Herculase Hotstart DNA Polymerase Instruction Manual

Herculase Hotstart DNA Polymerase is a heat-activated DNA polymerase for PCR and other DNA amplification applications.


Agilent Herculase Hotstart DNA Polymerase Instruction Manual - cover page
Brand
Agilent
Category
Other products
Document type
User Manual
Language
English
Pages
18
File format
PDF
File size
98 KB
Published
06 June, 2012
Updated
30 September, 2023
MD5 checksum
8C70074902446165EAD6B144AA2E682F

About this manual

This document serves as an instruction manual for the Agilent Herculase Hotstart DNA Polymerase (Catalog #600310, 600312, and 600314). It details the materials provided with each catalog number, including enzyme units, reaction buffer, and DMSO. The product is designed for in vitro use only and requires storage at -20°C.

What's inside

  • Materials Provided
  • Storage Conditions
  • Properties of Stratagene Hot-Start PCR Enzymes
  • Critical Optimization Parameters
  • DNA Template Quality and Concentration
  • Enzyme Concentration
  • DMSO Usage
  • Primer Design and Concentration
  • Deoxynucleotide Concentrations
  • Cycling Parameters

Specifications

Enzyme Activity (Catalog #600310)100 U
Enzyme Activity (Catalog #600312)500 U
Enzyme Activity (Catalog #600314)1000 U
Storage Temperature-20°C
Enzyme Concentration in Tube5 U/ul

Frequently asked questions

What is the maximum target length capability?

Herculase hotstart DNA polymerase can amplify genomic targets up to 37 kb and vector targets up to 48 kb.

What is the maximum length of genomic targets this polymerase can amplify?

The product can successfully amplify genomic targets up to 37 kb.

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